产品描述
This Human Salusin β ELISA Kit was designed for the quantitative measurement of Human Salusin β protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 pg/mL-30 pg/mL and the sensitivity is 0.11 pg/mL.
别名
Salusin Beta
缩写
Salusin β
灵敏度
0.11 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Salusin β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:100 | Average % | 83 | |
| Range % | 80-86 | |
| 1:200 | Average % | 102 | |
| Range % | 97-107 | |
| 1:400 | Average % | 110 | |
| Range % | 107-113 | |
| 1:800 | Average % | 99 | |
| Range % | 96-104 | |
回收率
| The recovery of human Salusin β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 82-93 | |
| EDTA plasma (n=4) | 100 | 95-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 30 | 2.598 | 2.574 | 2.586 | 2.491 | | | 15 | 2.201 | 2.235 | 2.218 | 2.123 | | | 7.5 | 1.596 | 1.568 | 1.582 | 1.487 | | | 3.75 | 0.903 | 0.935 | 0.919 | 0.824 | | | 1.8 | 0.466 | 0.475 | 0.471 | 0.376 | | | 0.9 | 0.327 | 0.338 | 0.333 | 0.238 | | | 0.45 | 0.225 | 0.241 | 0.233 | 0.138 | | | 0 | 0.094 | 0.096 | 0.095 | | |
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货期
3-5 working days