产品描述
PLA2G7是一种磷脂酶A2(PLA2)家族成员,它可以催化磷脂酰胆碱的水解反应,并释放出游离脂肪酸和溶血磷脂。PLA2G7的活性和含量的变化与心血管疾病、代谢紊乱和炎症等有关。因此,检测PLA2G7的水平可以帮助预测患者心血管疾病的风险,并有助于早期诊断和治疗。
华美生物所提供的Human lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人 血清、血浆、组织匀浆样本中的PLA2G7,其灵敏度为0.39 ng/mL,检测范围为1.56 ng/mL-100 ng/mL。
别名
Platelet-activating factor acetylhydrolase precursor ELISA Kit; 1 alkyl 2 acetylglycerophosphocholine esterase ELISA Kit; 1-alkyl-2-acetylglycerophosphocholine esterase ELISA Kit; 2 acetyl 1 alkylglycerophosphocholine esterase ELISA Kit; 2-acetyl-1-alkylglycerophosphocholine esterase ELISA Kit; EC 3.1.1.47 PAF acetylhydrolase PAF 2-acylhydrolase LDL associated phospholipase A2 ELISA Kit; Group VIIA phospholipase A2 ELISA Kit; Group-VIIA phospholipase A2 ELISA Kit; gVIIA PLA2 ELISA Kit; gVIIA-PLA2 ELISA Kit; LDL associated phospholipase A2 ELISA Kit; LDL PLA(2) ELISA Kit; LDL PLA2 ELISA Kit; LDL-associated phospholipase A2 ELISA Kit; LDL-PLA(2) ELISA Kit; Lipoprotein associated phospholipase A2 ELISA Kit; LP PLA2 ELISA Kit; PAF 2 acylhydrolase ELISA Kit; PAF 2 acylhydrolase LDL associated phospholipase A2 ELISA Kit; PAF 2-acylhydrolase ELISA Kit; PAF acetylhydrolase ELISA Kit; PAFA_HUMAN ELISA Kit; PAFAD ELISA Kit; PAFAH ELISA Kit; Phospholipase A2, group VII (platelet activating factor acetylhydrolase, plasma) ELISA Kit; PLA2G7 ELISA Kit; Platelet activating factor acetylhydrolase 2 ELISA Kit; Platelet activating factor acetylhydrolase ELISA Kit; Platelet-activating factor acetylhydrolase ELISA Kit
缩写
PLA2G7
Uniprot No
Q13093
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
93 |
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| Range % |
90-96 |
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| 1:10 |
Average % |
91 |
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| Range % |
87-96 |
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| 1:20 |
Average % |
97 |
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| Range % |
93-100 |
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| 1:40 |
Average % |
89 |
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| Range % |
85-96 |
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回收率
| The recovery of human Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
90-96 |
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| EDTA plasma (n=4) |
95 |
90-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.767 |
2.861 |
2.814 |
2.642 |
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| 50 |
2.356 |
2.313 |
2.335 |
2.163 |
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| 25 |
1.672 |
1.638 |
1.655 |
1.483 |
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| 12.5 |
0.983 |
0.972 |
0.978 |
0.806 |
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| 6.25 |
0.657 |
0.635 |
0.646 |
0.474 |
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| 3.12 |
0.455 |
0.444 |
0.450 |
0.278 |
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| 1.56 |
0.279 |
0.286 |
0.283 |
0.111 |
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| 0 |
0.170 |
0.173 |
0.172 |
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货期
3-5 working days