产品描述
This Pig S-100B ELISA Kit was designed for the quantitative measurement of Pig S-100B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL .
缩写
S-100B
Uniprot No
I3L5R6
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 91-100 | |
| 1:2 | Average % | 93 | |
| Range % | 85-101 | |
| 1:4 | Average % | 86 | |
| Range % | 80-98 | |
| 1:8 | Average % | 94 | |
| Range % | 92-105 | |
回收率
| The recovery of pig S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 83-102 | |
| EDTA plasma (n=4) | 95 | 92-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 5000 | 2.025 | 2.067 | 2.046 | 1.887 | | | 2500 | 1.574 | 1.524 | 1.549 | 1.390 | | | 1250 | 1.183 | 1.169 | 1.176 | 1.017 | | | 625 | 0.821 | 0.812 | 0.817 | 0.658 | | | 312 | 0.567 | 0.534 | 0.551 | 0.392 | | | 156 | 0.354 | 0.373 | 0.364 | 0.205 | | | 78 | 0.250 | 0.262 | 0.256 | 0.097 | | | 0 | 0.164 | 0.153 | 0.159 | | |
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货期
3-5 working days