产品描述
This Rabbit PG-E2 ELISA Kit was designed for the quantitative measurement of Rabbit PG-E2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .
缩写
PG-E2
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 80-98 | |
| 1:2 | Average % | 96 | |
| Range % | 91-100 | |
| 1:4 | Average % | 94 | |
| Range % | 90-105 | |
| 1:8 | Average % | 97 | |
| Range % | 86-98 | |
回收率
| The recovery of rabbit PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 85-110 | |
| EDTA plasma (n=4) | 90 | 80-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.913 | 2.812 | 2.863 | 2.703 | | | 1000 | 2.138 | 2.038 | 2.088 | 1.928 | | | 500 | 1.157 | 1.098 | 1.128 | 0.968 | | | 250 | 0.615 | 0.598 | 0.607 | 0.447 | | | 125 | 0.372 | 0.347 | 0.360 | 0.200 | | | 62.5 | 0.282 | 0.272 | 0.277 | 0.117 | | | 31.25 | 0.203 | 0.187 | 0.195 | 0.035 | | | 0 | 0.163 | 0.157 | 0.160 | | |
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货期
3-5 working days