产品描述

This Human TPT1 ELISA Kit was designed for the quantitative measurement of Human TPT1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

别名

FLJ27337 ELISA Kit; Fortilin ELISA Kit; Histamine releasing factor ELISA Kit; Histamine-releasing factor ELISA Kit; HRF ELISA Kit; Immunoglobulin E-dependent ELISA Kit; p02 ELISA Kit; p23 ELISA Kit; TCTP ELISA Kit; TCTP_HUMAN ELISA Kit; tpt1 ELISA Kit; Translationally controlled tumor protein ELISA Kit; Translationally-controlled tumor protein ELISA Kit; Tumor protein translationally controlled 1 ELISA Kit; tumor protein; translationally-controlled 1 ELISA Kit

缩写

TPT1

Uniprot No

P13693

灵敏度

3.12 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-97
1:2		Average %		97
		Range %		93-103
1:4		Average %		84
		Range %		81-89
1:8		Average %		89
		Range %		83-95

回收率

The recovery of human TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		102		97-107
EDTA plasma (n=4)		92		89-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 800 2.352 2.236 2.294 2.161 400 1.799 1.777 1.788 1.655 200 1.218 1.255 1.237 1.104 100 0.679 0.709 0.694 0.561 50 0.454 0.480 0.467 0.334 25 0.245 0.236 0.241 0.108 12.5 0.202 0.205 0.204 0.071 0 0.127 0.138 0.133

货期

3-5 working days