产品描述

This Human SERPINB5 ELISA Kit was designed for the quantitative measurement of Human SERPINB5 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

别名

Maspin ELISA Kit; Ovalbumin ELISA Kit; Peptidase inhibitor 5 ELISA Kit; PI-5 ELISA Kit; PI5 ELISA Kit; protease inhibitor 5 (maspin) ELISA Kit; Protease inhibitor 5 ELISA Kit; Serine (or cysteine) peptidase inhibitor; clade B; member 5 ELISA Kit; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 5 ELISA Kit; Serpin B5 ELISA Kit; serpin family B member 5 ELISA Kit; Serpin peptidase inhibitor ELISA Kit; Serpin peptidase inhibitor; clade B (ovalbumin); member 5 ELISA Kit; Serpinb5 ELISA Kit; SPB5_HUMAN ELISA Kit; Spi5 ELISA Kit; Spi7 ELISA Kit

缩写

SERPINB5

Uniprot No

P36952

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		86-93
1:2		Average %		100
		Range %		97-102
1:4		Average %		94
		Range %		89-97
1:8		Average %		97
		Range %		91-100

回收率

The recovery of human SERPINB5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-97
EDTA plasma (n=4)		97		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.286 2.226 2.256 2.074 5 1.876 1.846 1.861 1.679 2.5 1.562 1.550 1.556 1.374 1.25 1.157 1.142 1.150 0.968 0.625 0.718 0.729 0.724 0.542 0.312 0.480 0.498 0.489 0.307 0.156 0.318 0.324 0.321 0.139 0 0.181 0.183 0.182

货期

3-5 working days