产品描述
This Rat PPARG ELISA Kit was designed for the quantitative measurement of Rat PPARG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
Pparg ELISA Kit; Nr1c3 ELISA Kit; Peroxisome proliferator-activated receptor gamma ELISA Kit; PPAR-gamma ELISA Kit; Nuclear receptor subfamily 1 group C member 3 ELISA Kit
缩写
PPARG
Uniprot No
O88275
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PPAR-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-103 | |
| 1:2 | Average % | 90 | |
| Range % | 85-94 | |
| 1:4 | Average % | 104 | |
| Range % | 100-109 | |
| 1:8 | Average % | 84 | |
| Range % | 80-88 | |
回收率
| The recovery of rat PPAR-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 85-95 | |
| EDTA plasma (n=4) | 98 | 93-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.371 | 2.303 | 2.337 | 2.239 | | | 5 | 1.712 | 1.826 | 1.769 | 1.671 | | | 2.5 | 1.157 | 1.114 | 1.136 | 1.038 | | | 1.25 | 0.604 | 0.576 | 0.590 | 0.492 | | | 0.625 | 0.312 | 0.327 | 0.320 | 0.222 | | | 0.312 | 0.179 | 0.169 | 0.174 | 0.076 | | | 0.156 | 0.115 | 0.109 | 0.112 | 0.014 | | | 0 | 0.094 | 0.101 | 0.098 | | |
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货期
3-5 working days