产品描述
This Pig TCC C5b-9 ELISA Kit was designed for the quantitative measurement of Pig TCC C5b-9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 ng/mL-1200 ng/mL and the sensitivity is 4.69 ng/mL.
缩写
TCC C5b-9
灵敏度
4.69 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 99 | |
| Range % | 85-105 | |
| 1:2 | Average % | 95 | |
| Range % | 85-113 | |
| 1:4 | Average % | 95 | |
| Range % | 82-102 | |
| 1:8 | Average % | 97 | |
| Range % | 85-110 | |
回收率
| The recovery of pig TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 88-109 | |
| EDTA plasma (n=4) | 97 | 85-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 1200 | 2.217 | 2.319 | 2.268 | 2.130 | | | 600 | 1.790 | 1.767 | 1.779 | 1.641 | | | 300 | 1.123 | 1.132 | 1.128 | 0.990 | | | 150 | 0.675 | 0.651 | 0.663 | 0.525 | | | 75 | 0.397 | 0.403 | 0.400 | 0.262 | | | 37.5 | 0.285 | 0.298 | 0.292 | 0.154 | | | 18.75 | 0.231 | 0.216 | 0.224 | 0.086 | | | 0 | 0.141 | 0.134 | 0.138 | | |
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货期
3-5 working days