产品描述

This Human SCYE1 ELISA Kit was designed for the quantitative measurement of Human SCYE1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

AIMP 1 ELISA Kit; Aimp1 ELISA Kit; AIMP1/p43 ELISA Kit; AIMP1_HUMAN ELISA Kit; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 ELISA Kit; ARS interacting multifunctional protein 1 ELISA Kit; EMAP 2 ELISA Kit; EMAP-2 ELISA Kit; EMAP-II ELISA Kit; EMAP2 ELISA Kit; EMAPII ELISA Kit; Endothelial monocyte activating polypeptide 2 ELISA Kit; Endothelial monocyte activating polypeptide ELISA Kit; Endothelial monocyte-activating polypeptide 2 ELISA Kit; Endothelial monocyte-activating polypeptide II ELISA Kit; HLD3 ELISA Kit; Multisynthase complex auxiliary component p43 ELISA Kit; Multisynthetase complex auxiliary component p43 ELISA Kit; OTTHUMP00000219673 ELISA Kit; p43 ELISA Kit; Scye 1 ELISA Kit; Scye1 ELISA Kit; Scye1, formerly ELISA Kit; Small inducible cytokine subfamily E member 1 ELISA Kit; Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) ELISA Kit; Small inducible cytokine subfamily E, member 1, formerly ELISA Kit

缩写

SCYE1

Uniprot No

Q12904

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SCYE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		102
		Range %		95-106
1:2		Average %		101
		Range %		96-105
1:4		Average %		89
		Range %		86-94
1:8		Average %		94
		Range %		89-98

回收率

The recovery of human SCYE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		104		98-108
EDTA plasma (n=4)		93		86-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1500 1.976 1.933 1.955 1.800 750 1.365 1.284 1.325 1.170 375 0.872 0.828 0.850 0.695 187.5 0.578 0.545 0.562 0.407 94 0.388 0.361 0.375 0.220 47 0.251 0.246 0.249 0.094 23.5 0.219 0.215 0.217 0.062 0 0.156 0.154 0.155

货期

3-5 working days