产品描述
This Human PF4V1 ELISA Kit was designed for the quantitative measurement of Human PF4V1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
别名
PF4V1 ELISA Kit; CXCL4V1 ELISA Kit; SCYB4V1 ELISA Kit; Platelet factor 4 variant ELISA Kit; C-X-C motif chemokine 4 variant ELISA Kit; CXCL4L1 ELISA Kit; PF4alt ELISA Kit; PF4var1) [Cleaved into: Platelet factor 4 variant(4-74); Platelet factor 4 variant(5-74); Platelet factor 4 variant(6-74)] ELISA Kit
缩写
PF4V1
Uniprot No
P10720
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PF4V1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 89-99 | |
| 1:2 | Average % | 98 | |
| Range % | 95-103 | |
| 1:4 | Average % | 102 | |
| Range % | 94-106 | |
| 1:8 | Average % | 95 | |
| Range % | 90-98 | |
回收率
| The recovery of human PF4V1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 89-95 | |
| EDTA plasma (n=4) | 99 | 96-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.945 | 3.094 | 3.020 | 2.831 | | | 20 | 2.266 | 2.399 | 2.333 | 2.144 | | | 10 | 1.532 | 1.497 | 1.515 | 1.326 | | | 5 | 0.775 | 0.776 | 0.776 | 0.587 | | | 2.5 | 0.453 | 0.454 | 0.454 | 0.265 | | | 1.25 | 0.369 | 0.341 | 0.355 | 0.166 | | | 0.625 | 0.230 | 0.250 | 0.240 | 0.051 | | | 0 | 0.184 | 0.194 | 0.189 | | |
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货期
3-5 working days