产品描述
This Human SCGB2A1 ELISA Kit was designed for the quantitative measurement of Human SCGB2A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.235 ng/mL-15 ng/mL and the sensitivity is 0.058 ng/mL.
别名
Lacryglobin ELISA Kit; LIPHC ELISA Kit; Lipophilin C ELISA Kit; Lipophilin-C ELISA Kit; LPHC ELISA Kit; LPNC ELISA Kit; Mammaglobin 2 ELISA Kit; Mammaglobin-2 ELISA Kit; Mammaglobin-B ELISA Kit; MGB2 ELISA Kit; MGC71973 ELISA Kit; Scgb2a1 ELISA Kit; Secretoglobin family 2A member 1 ELISA Kit; Secretoglobin; family 2A; member 1 ELISA Kit; SG2A1_HUMAN ELISA Kit; UGB3 ELISA Kit
缩写
SCGB2A1
Uniprot No
O75556
灵敏度
0.058 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB2A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-99 | |
| 1:2 | Average % | 100 | |
| Range % | 95-106 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 85 | |
| Range % | 80-89 | |
回收率
| The recovery of human SCGB2A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 93-102 | |
| EDTA plasma (n=4) | 92 | 88-87 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 15 | 2.345 | 2.252 | 2.299 | 2.158 | | | 7.5 | 1.723 | 1.676 | 1.700 | 1.559 | | | 3.75 | 1.215 | 1.154 | 1.185 | 1.044 | | | 1.88 | 0.721 | 0.743 | 0.732 | 0.591 | | | 0.94 | 0.436 | 0.441 | 0.439 | 0.298 | | | 0.47 | 0.302 | 0.288 | 0.295 | 0.154 | | | 0.235 | 0.234 | 0.219 | 0.227 | 0.086 | | | 0 | 0.143 | 0.139 | 0.141 | | |
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货期
3-5 working days