产品描述
This Human UTS2 ELISA Kit was designed for the quantitative measurement of Human UTS2 protein in serum, saliva, cerebrospinal fluid (CSF), tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
PRO1068 ELISA Kit; U-II ELISA Kit; UCN2 ELISA Kit; UII ELISA Kit; Urotensin 2 preproprotein isoform a ELISA Kit; Urotensin 2 preproprotein isoform b ELISA Kit; Urotensin II ELISA Kit; Urotensin-2 ELISA Kit; UTS2 ELISA Kit; UTS2_HUMAN ELISA Kit; UTSII ELISA Kit
缩写
UTS2
Uniprot No
O95399
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human urotensinⅡ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 90-106 | |
| 1:2 | Average % | 94 | |
| Range % | 86-98 | |
| 1:4 | Average % | 87 | |
| Range % | 82-96 | |
| 1:8 | Average % | 91 | |
| Range % | 95-97 | |
回收率
| The recovery of human urotensinⅡ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.930 | 2.898 | 2.914 | 2.809 | | | 10 | 2.160 | 2.008 | 2.084 | 1.979 | | | 5 | 1.565 | 1.482 | 1.524 | 1.419 | | | 2.5 | 1.005 | 0.999 | 1.002 | 0.897 | | | 1.25 | 0.517 | 0.512 | 0.515 | 0.410 | | | 0.625 | 0.337 | 0.321 | 0.329 | 0.224 | | | 0.312 | 0.247 | 0.228 | 0.238 | 0.133 | | | 0 | 0.107 | 0.103 | 0.105 | | |
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货期
3-5 working days