产品描述

This Human PTTG1 ELISA Kit was designed for the quantitative measurement of Human PTTG1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.2 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

AW555095 ELISA Kit; C87862 ELISA Kit; Cut2 ELISA Kit; EAP 1 ELISA Kit; EAP1 ELISA Kit; ESP1 associated protein 1 ELISA Kit; Esp1-associated protein ELISA Kit; hPTTG ELISA Kit; MGC126883 ELISA Kit; MGC138276 ELISA Kit; Pds1 ELISA Kit; Pituitary tumor transforming 1 ELISA Kit; Pituitary tumor transforming protein 1 ELISA Kit; Pituitary tumor-transforming 1; isoform CRA_a ELISA Kit; Pituitary tumor-transforming 1; isoform CRA_b ELISA Kit; Pituitary tumor-transforming gene 1 ELISA Kit; Pituitary tumor-transforming gene 1 protein ELISA Kit; PTTG 1 ELISA Kit; PTTG ELISA Kit; PTTG1 ELISA Kit; PTTG1 protein ELISA Kit; PTTG1_HUMAN ELISA Kit; Pttg3 ELISA Kit; Securin ELISA Kit; Tumor transforming 1 ELISA Kit; Tumor transforming protein 1 ELISA Kit; Tumor-transforming protein 1 ELISA Kit; TUTR 1 ELISA Kit; TUTR1 ELISA Kit

缩写

PTTG1

Uniprot No

O95997

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PTTG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		86-97
1:2		Average %		107
		Range %		103-112
1:4		Average %		88
		Range %		83-94
1:8		Average %		86
		Range %		81-92

回收率

The recovery of human PTTG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		106		102-110
EDTA plasma (n=4)		89		86-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     2000 2.027 2.156 2.092 1.962     1000 1.679 1.778 1.729 1.599     500 1.173 1.213 1.193 1.063     250 0.715 0.722 0.719 0.589     125 0.445 0.458 0.452 0.322     62.5 0.305 0.319 0.312 0.182     31.2 0.221 0.227 0.224 0.094     0 0.129 0.131 0.130

货期

3-5 working days