产品描述
This Bovine SFTPB ELISA Kit was designed for the quantitative measurement of Bovine SFTPB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.37 ng/mL-600 ng/mL and the sensitivity is 2.34 ng/mL.
别名
SFTPB ELISA Kit; SFTP3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; 6 kDa protein ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
缩写
SFTPB
Uniprot No
P15781
灵敏度
2.34 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-103 | |
| 1:2 | Average % | 91 | |
| Range % | 85-97 | |
| 1:4 | Average % | 90 | |
| Range % | 88-94 | |
| 1:8 | Average % | 100 | |
| Range % | 96-105 | |
回收率
| The recovery of bovine SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 87-93 | |
| EDTA plasma (n=4) | 106 | 103-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 600 | 2.238 | 2.412 | 2.325 | 2.191 | | | 300 | 1.603 | 1.620 | 1.612 | 1.478 | | | 150 | 1.054 | 1.102 | 1.078 | 0.944 | | | 75 | 0.766 | 0.771 | 0.769 | 0.635 | | | 37.5 | 0.534 | 0.580 | 0.557 | 0.423 | | | 18.75 | 0.322 | 0.329 | 0.326 | 0.192 | | | 9.37 | 0.234 | 0.219 | 0.227 | 0.093 | | | 0 | 0.132 | 0.135 | 0.134 | | |
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货期
3-5 working days