产品描述

This Human THPO ELISA Kit was designed for the quantitative measurement of Human THPO protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

别名

C mpl ligand ELISA Kit; C-mpl ligand ELISA Kit; Megakaryocyte colony stimulating factor ELISA Kit; Megakaryocyte colony-stimulating factor ELISA Kit; Megakaryocyte growth and development factor ELISA Kit; Megakaryocyte stimulating factor ELISA Kit; MGC163194 ELISA Kit; MGDF ELISA Kit; MKCSF ELISA Kit; ML ELISA Kit; MPL ligand ELISA Kit; MPLLG ELISA Kit; Myeloproliferative leukemia virus oncogene ligand ELISA Kit; Prepro thrombopoietin ELISA Kit; THCYT1 ELISA Kit; THPO ELISA Kit; Thrombopoietin ELISA Kit; Thrombopoietin nirs variant 1 ELISA Kit; TPO ELISA Kit; TPO_HUMAN ELISA Kit

缩写

THPO

Uniprot No

P40225

灵敏度

1.56 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		90-101
1:2		Average %		97
		Range %		91-102
1:4		Average %		95
		Range %		90-100
1:8		Average %		94
		Range %		89-99

回收率

The recovery of human TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		92-102
EDTA plasma (n=4)		88		82-93

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 400 2.664 2.572 2.618 2.483 200 2.085 2.178 2.132 1.997 100 1.489 1.394 1.442 1.307 50 0.822 0.842 0.832 0.697 25 0.433 0.457 0.445 0.310 12.5 0.293 0.284 0.289 0.154 6.25 0.196 0.198 0.197 0.062 0 0.134 0.135 0.135

货期

3-5 working days