产品描述
Visceral Adipose-Specific Serine Protease Inhibitor, Vaspin is a member of the serine protease inhibitor family which has insulin-sensitizing effects.The Rat Visceral Adipose-Specific Serine Protease Inhibitor, Vaspin ELISA Kit is an essential tool for researchers in the metabolism and cardiovascular field. This quantitative assay is designed to detect and measure the level of Visceral Adipose Tissue-Derived Serine Protease Inhibitor (Vaspin) in serum, plasma, and tissue homogenates of Rattus norvegicus (Rat).
The assay principle of this kit is based on the sandwich method, which ensures accurate and reliable results. The detection range of the Rat Vaspin ELISA Kit is 31.25 pg/mL-2000 pg/mL, and the sensitivity is 7.8 pg/mL. The assay time for this kit is 1-5 hours, and the sample volume required is only 50-100 ul.
This kit's measurement is conducted at a detection wavelength of 450 nm, allowing for precise and efficient detection of Vaspin levels. Researchers can confidently use this kit to investigate the potential role of Vaspin in metabolism and cardiovascular diseases, given its specificity for visceral adipose tissue.
别名
Serpina12 ELISA Kit; Serpin A12 ELISA Kit; Visceral adipose tissue-derived serine protease inhibitor ELISA Kit; Vaspin ELISA Kit; Visceral adipose-specific serpin ELISA Kit
缩写
SERPINA12
Uniprot No
Q8R4Z1
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat vaspin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 80-90 | |
| 1:2 | Average % | 94 | |
| Range % | 90-98 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 97 | |
| Range % | 92-104 | |
回收率
| The recovery of rat vaspin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 105 | 100-110 | |
| EDTA plasma (n=4) | 95 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.679 | 2.576 | 2.628 | 2.479 | | | 1000 | 2.071 | 2.114 | 2.093 | 1.944 | | | 500 | 1.582 | 1.472 | 1.527 | 1.378 | | | 250 | 0.958 | 0.913 | 0.936 | 0.787 | | | 125 | 0.580 | 0.589 | 0.585 | 0.436 | | | 62.5 | 0.412 | 0.407 | 0.410 | 0.261 | | | 31.25 | 0.302 | 0.279 | 0.291 | 0.142 | | | 0 | 0.147 | 0.151 | 0.149 | | |
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货期
3-5 working days
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