产品描述

This Human SMARCA4 ELISA Kit was designed for the quantitative measurement of Human SMARCA4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

ATP dependent helicase SMARCA4 ELISA Kit; ATP-dependent helicase SMARCA4 ELISA Kit; BAF 190 ELISA Kit; BAF190 ELISA Kit; BAF190A ELISA Kit; Brahma protein like 1 ELISA Kit; BRG1 ELISA Kit; BRG1 associated factor 190A ELISA Kit; BRG1 protein ELISA Kit; BRG1-associated factor 190A ELISA Kit; BRM/SWI2 related gene 1 ELISA Kit; Global transcription activator homologous sequence ELISA Kit; global transcription activator snf2l4 ELISA Kit; Homeotic gene regulator ELISA Kit; hSNF2b ELISA Kit; Mitotic growth and transcription activator ELISA Kit; MRD16 ELISA Kit; Nuclear protein GRB1 ELISA Kit; Protein brahma homolog 1 ELISA Kit; Protein BRG-1 ELISA Kit; Protein BRG1 ELISA Kit; RTPS2 ELISA Kit; SMARC A4 ELISA Kit; SMARCA4 ELISA Kit; SMCA4_HUMAN ELISA Kit; SNF2 ELISA Kit; SNF2 beta ELISA Kit; SNF2 like 4 ELISA Kit; SNF2-beta ELISA Kit; SNF2B ELISA Kit; SNF2L4 ELISA Kit; SNF2LB ELISA Kit; Sucrose nonfermenting like 4 ELISA Kit; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 ELISA Kit; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 ELISA Kit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 ELISA Kit; SWI2 ELISA Kit; Transcription activator BRG1 ELISA Kit

缩写

SMARCA4

Uniprot No

P51532

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SMARCA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		82-91
1:2		Average %		104
		Range %		98-107
1:4		Average %		94
		Range %		88-98
1:8		Average %		92
		Range %		88-96

回收率

The recovery of human SMARCA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		86-95
EDTA plasma (n=4)		89		87-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1600 2.965 2.909 2.937 2.763 800 2.342 2.215 2.279 2.105 400 1.495 1.462 1.479 1.305 200 0.914 0.900 0.907 0.733 100 0.567 0.555 0.561 0.387 50 0.378 0.363 0.371 0.197 25 0.248 0.243 0.246 0.072 0 0.175 0.173 0.174

货期

3-5 working days