产品描述
This Human PIVKA-II ELISA Kit was designed for the quantitative measurement of Human PIVKA-II protein in serum, plasma, ascitic fluid, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
缩写
PIVKA-II
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PIVKA-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
94 |
|
| Range % |
90-101 |
|
| 1:2 |
Average % |
97 |
|
| Range % |
86-101 |
|
| 1:4 |
Average % |
96 |
|
| Range % |
90-108 |
|
| 1:8 |
Average % |
94 |
|
| Range % |
82-103 |
|
回收率
| The recovery of human PIVKA-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-104 |
|
| EDTA plasma (n=4) |
97 |
91-105 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.467 |
2.418 |
2.443 |
2.327 |
|
| 10 |
1.962 |
1.945 |
1.954 |
1.838 |
|
| 5 |
1.342 |
1.311 |
1.327 |
1.211 |
|
| 2.5 |
0.823 |
0.772 |
0.798 |
0.682 |
|
| 1.25 |
0.504 |
0.496 |
0.500 |
0.384 |
|
| 0.625 |
0.331 |
0.321 |
0.326 |
0.210 |
|
| 0.312 |
0.201 |
0.189 |
0.195 |
0.079 |
|
| 0 |
0.117 |
0.114 |
0.116 |
|
|
|
货期
3-5 working days