产品描述
This Human TGFB3 ELISA Kit was designed for the quantitative measurement of Human TGFB3 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
ARVD ELISA Kit; ARVD1 ELISA Kit; FLJ16571 ELISA Kit; LDS5 ELISA Kit; MGC105479 ELISA Kit; MGC118722 ELISA Kit; prepro-transforming growth factor beta-3 ELISA Kit; RNHF ELISA Kit; TGF beta 3 ELISA Kit; TGF beta3 ELISA Kit; TGF-beta-3 ELISA Kit; TGFB 3 ELISA Kit; Tgfb3 ELISA Kit; TGFB3_HUMAN ELISA Kit; transforming growth factor beta 3 ELISA Kit; Transforming growth factor beta-3 ELISA Kit
缩写
TGFB3
Uniprot No
P10600
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-95 |
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| 1:2 |
Average % |
97 |
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| Range % |
94-103 |
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| 1:4 |
Average % |
98 |
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| Range % |
90-104 |
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| 1:8 |
Average % |
87 |
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| Range % |
82-91 |
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回收率
| The recovery of human TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-98 |
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| EDTA plasma (n=4) |
98 |
96-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.699 |
2.752 |
2.726 |
2.571 |
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| 500 |
2.435 |
2.399 |
2.417 |
2.262 |
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| 250 |
1.808 |
1.875 |
1.842 |
1.687 |
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| 125 |
1.065 |
1.055 |
1.060 |
0.905 |
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| 62.5 |
0.678 |
0.735 |
0.707 |
0.552 |
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| 31.2 |
0.401 |
0.422 |
0.412 |
0.257 |
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| 15.6 |
0.267 |
0.275 |
0.271 |
0.116 |
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| 0 |
0.149 |
0.160 |
0.155 |
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货期
3-5 working days