产品描述
T4(甲状腺素)是人体内的甲状腺激素之一,对调节基础代谢率、心脏、神经、肌肉和消化等功能有重要作用。甲状腺合成的大部分是T4,只有少量被代谢成活性更高的T3。T4的含量受到下丘脑-垂体-甲状腺轴的调节,血液中的T4含量反映了甲状腺的功能状态,可用于甲状腺疾病的诊断和治疗。
华美生物所提供的Guinea pig thyroxine (T4) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测豚鼠血清、血浆样本中的T4,其灵敏度为20 ng/mL,检测范围为20 ng/mL-320 ng/mL。
缩写
T4
灵敏度
20 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
85-100 |
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| 1:2 |
Average % |
90 |
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| Range % |
81-99 |
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| 1:4 |
Average % |
92 |
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| Range % |
85-100 |
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| 1:8 |
Average % |
90 |
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| Range % |
90-100 |
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回收率
| The recovery of Guinea pig T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
86-99 |
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| EDTA plasma (n=4) |
93 |
85-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 320 |
0.175 |
0.171 |
0.173 |
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| 160 |
0.275 |
0.280 |
0.278 |
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| 80 |
0.502 |
0.493 |
0.498 |
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| 40 |
1.006 |
1.028 |
1.017 |
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| 20 |
2.024 |
2.049 |
2.037 |
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货期
3-5 working days