产品描述
This Mouse SERPINE1 ELISA Kit was designed for the quantitative measurement of Mouse SERPINE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.22 ng/mL.
别名
Serpine1 ELISA Kit; Mr1 ELISA Kit; Pai1 ELISA Kit; Planh1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
缩写
SERPINE1
Uniprot No
P22777
灵敏度
0.22 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
94 |
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| Range % |
88-102 |
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| 1:8 |
Average % |
96 |
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| Range % |
91-103 |
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回收率
| The recovery of mouse PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-101 |
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| EDTA plasma (n=4) |
95 |
92-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 60 |
3.033 |
3.108 |
3.071 |
2.894 |
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| 30 |
2.476 |
2.557 |
2.517 |
2.340 |
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| 15 |
1.887 |
1.785 |
1.836 |
1.659 |
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| 7.5 |
1.117 |
1.124 |
1.121 |
0.944 |
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| 3.75 |
0.711 |
0.719 |
0.715 |
0.538 |
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| 1.8 |
0.488 |
0.468 |
0.478 |
0.301 |
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| 0.9 |
0.329 |
0.332 |
0.331 |
0.154 |
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| 0 |
0.178 |
0.176 |
0.177 |
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货期
3-5 working days