别名
4732496E14Rik ELISA Kit; EBI6 ELISA Kit; EBV induced protein 6 ELISA Kit; Epstein-Barr virus-induced protein 6 ELISA Kit; MGC:10353 ELISA Kit; Tnf receptor associated factor 1 ELISA Kit; TNF receptor-associated factor 1 ELISA Kit; TRAF 1 ELISA Kit; TRAF1 ELISA Kit; TRAF1_HUMAN ELISA Kit
缩写
TRAF1
Uniprot No
Q13077
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRAF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 82-90 | | |
| 1:2 | Average % | 101 | | |
| Range % | 98-106 | | |
| 1:4 | Average % | 92 | | |
| Range % | 85-96 | | |
| 1:8 | Average % | 94 | | |
| Range % | 90-98 | | |
回收率
| The recovery of human TRAF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 86-94 | | |
| EDTA plasma (n=4) | 107 | 103-112 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 2000 | 2.421 | 2.568 | 2.495 | 2.397 | | | | 1000 | 1.858 | 1.902 | 1.880 | 1.782 | | | | 500 | 1.224 | 1.277 | 1.251 | 1.153 | | | | 250 | 0.694 | 0.705 | 0.700 | 0.602 | | | | 125 | 0.432 | 0.443 | 0.438 | 0.340 | | | | 62.5 | 0.246 | 0.252 | 0.249 | 0.151 | | | | 31.25 | 0.162 | 0.166 | 0.164 | 0.066 | | | | 0 | 0.097 | 0.099 | 0.098 | | | |
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货期
3-5 working days