产品描述
The Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ELISA Kit, a highly sensitive and specific assay for the quantitative detection of UCHL1 in serum, plasma, tissue homogenates, and cell lysates from the Rat species.
UCHL1,plays an important role in ubiquitin-proteasome system, is a protein that plays a crucial role in the regulation of cellular processes such as protein degradation and cell signaling. Dysregulation of UCHL1 has been implicated in various diseases, including Parkinson's disease and cancer. Our UCHL1 ELISA Kit offers a reliable and efficient means of measuring UCHL1 levels in biological samples, with a detection range of 0.78 ng/mL to 50 ng/mL and a sensitivity of 0.195 ng/mL.
The assay principle of our UCHL1 ELISA Kit is based on the sandwich method. During the assay process, UCHL1 in the sample is sandwiched between two UCHL1-specific antibodies, which are coated on the plate. The bound UCHL1 is then detected using a detection antibody conjugated with HRP and a chromogenic substrate, producing a colorimetric signal that is directly proportional to the UCHL1 concentration.
This UCHL1 ELISA Kit offers a quick assay time of 1-5 hours, with a sample volume of 50-100ul and a detection wavelength of 450 nm. It is suitable for use in cell biology research and has been optimized for use with Rat samples. Its quantitative assay principle, coupled with its wide detection range and high sensitivity, make it an invaluable tool for researchers studying the physiological and pathological roles of UCHL1 in cellular processes.
别名
Uchl1 ELISA Kit; Ubiquitin carboxyl-terminal hydrolase isozyme L1 ELISA Kit; UCH-L1 ELISA Kit; EC 3.4.19.12 ELISA Kit; Neuron cytoplasmic protein 9.5 ELISA Kit; PGP 9.5 ELISA Kit; PGP9.5 ELISA Kit; Ubiquitin thioesterase L1 ELISA Kit
缩写
UCHL1
Uniprot No
Q00981
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat UCHL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 84-93 | |
| 1:2 | Average % | 101 | |
| Range % | 98-106 | |
| 1:4 | Average % | 89 | |
| Range % | 85-93 | |
| 1:8 | Average % | 104 | |
| Range % | 100-108 | |
回收率
| The recovery of rat UCHL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 85 | 80-90 | |
| EDTA plasma (n=4) | 96 | 93-99 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.196 | 2.174 | 2.185 | 2.050 | | | 25 | 1.618 | 1.635 | 1.627 | 1.492 | | | 12.5 | 1.071 | 1.098 | 1.085 | 0.950 | | | 6.25 | 0.682 | 0.654 | 0.668 | 0.533 | | | 3.12 | 0.450 | 0.436 | 0.443 | 0.308 | | | 1.56 | 0.356 | 0.368 | 0.362 | 0.227 | | | 0.78 | 0.251 | 0.265 | 0.258 | 0.123 | | | 0 | 0.134 | 0.136 | 0.135 | | |
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
