产品描述

This Human PDGFC ELISA Kit was designed for the quantitative measurement of Human PDGFC protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

别名

Fallotein ELISA Kit; hSCDGF ELISA Kit; PDGF-C ELISA Kit; PDGFC ELISA Kit; PDGFC latent form ELISA Kit; PDGFC receptor-binding form ELISA Kit; PDGFC_HUMAN ELISA Kit; Platelet derived growth factor C ELISA Kit; Platelet-derived growth factor C; receptor-binding form ELISA Kit; SCDGF ELISA Kit; Secretory growth factor like protein ELISA Kit; Secretory growth factor like protein fallotein ELISA Kit; Spinal cord derived growth factor ELISA Kit; Spinal cord-derived growth factor ELISA Kit; VEGF E ELISA Kit; VEGF-E ELISA Kit

缩写

PDGFC

Uniprot No

Q9NRA1

灵敏度

1.56 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-90
1:2		Average %		96
		Range %		90-100
1:4		Average %		98
		Range %		91-105
1:8		Average %		90
		Range %		82-98

回收率

The recovery of human VEGF-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		83-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 400 2.806 2.712 2.759 2.659 200 2.012 2.066 2.039 1.939 100 1.473 1.384 1.429 1.329 50 0.958 0.893 0.926 0.826 25 0.494 0.473 0.484 0.384 12.5 0.321 0.301 0.311 0.211 6.25 0.254 0.249 0.252 0.152 0 0.102 0.097 0.100

货期

3-5 working days