产品描述
TNFRSF1A是一种由TNFRSF1A基因编码的蛋白质,是TNF-α的高亲和力受体,具有重要的生物学功能。TNFRSF1A可与TNF-α结合并促进炎症反应、免疫细胞增殖以及凋亡信号的传递。
华美生物所提供的Human Tumor necrosis factor soluble receptor Ⅰ,TNFsR-ⅠELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆、尿液样本中的TNFRSF1A,其灵敏度为65 pg/mL,检测范围为78.125 pg/mL-5000 pg/mL。
别名
CD120a ELISA Kit; FPF ELISA Kit; MGC19588 ELISA Kit; p55 ELISA Kit; p55-R ELISA Kit; p60 ELISA Kit; TBP1 ELISA Kit; TBPI ELISA Kit; TNF R ELISA Kit; TNF R55 ELISA Kit; TNF-R1 ELISA Kit; TNF-RI ELISA Kit; TNFAR ELISA Kit; TNFR-I ELISA Kit; TNFR1 ELISA Kit; TNFR55 ELISA Kit; TNFR60 ELISA Kit; TNFRI ELISA Kit; TNFRSF1a ELISA Kit; TNR1A_HUMAN ELISA Kit; Tumor necrosis factor receptor 1 ELISA Kit; Tumor necrosis factor receptor superfamily, member 1A ELISA Kit; Tumor necrosis factor receptor type 1 ELISA Kit; Tumor necrosis factor receptor type I ELISA Kit; Tumor necrosis factor-binding protein 1 ELISA Kit
缩写
TNFRSF1A
Uniprot No
P19438
灵敏度
65 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNFsR-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
92-105 |
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| 1:2 |
Average % |
91 |
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| Range % |
86-98 |
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| 1:4 |
Average % |
91 |
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| Range % |
88-96 |
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| 1:8 |
Average % |
101 |
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| Range % |
96-106 |
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回收率
| The recovery of human TNFsR-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
87-93 |
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| EDTA plasma (n=4) |
101 |
103-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.568 |
2.632 |
2.600 |
2.450 |
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| 2500 |
2.245 |
2.121 |
2.183 |
2.033 |
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| 1250 |
1.622 |
1.741 |
1.682 |
1.532 |
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| 625 |
0.977 |
0.955 |
0.966 |
0.816 |
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| 312.5 |
0.660 |
0.626 |
0.643 |
0.493 |
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| 156.25 |
0.392 |
0.366 |
0.379 |
0.229 |
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| 78.125 |
0.261 |
0.254 |
0.258 |
0.108 |
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| 0 |
0.145 |
0.154 |
0.150 |
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货期
3-5 working days