产品描述
This Rat TNFsR2 ELISA Kit was designed for the quantitative measurement of Rat TNFsR2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
别名
Tnfrsf1b ELISA Kit; Tnfr2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; TNF-R2 ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; TNF-RII ELISA Kit; TNFR-II ELISA Kit; p75 ELISA Kit; p80 TNF-alpha receptor ELISA Kit; CD antigen CD120b ELISA Kit
缩写
TNFsR-
Uniprot No
Q80WY6
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 91 | | |
| Range % | 87-94 | | |
| 1:2 | Average % | 97 | | |
| Range % | 92-101 | | |
| 1:4 | Average % | 90 | | |
| Range % | 87-98 | | |
| 1:8 | Average % | 92 | | |
| Range % | 85-95 | | |
回收率
| The recovery of rat TNFsR-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 93 | 88-96 | | |
| EDTA plasma (n=4) | 98 | 95-102 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 40 | 2.827 | 2.899 | 2.863 | 2.732 | | | | 20 | 2.222 | 2.323 | 2.273 | 2.142 | | | | 10 | 1.373 | 1.412 | 1.393 | 1.262 | | | | 5 | 0.816 | 0.866 | 0.841 | 0.710 | | | | 2.5 | 0.531 | 0.545 | 0.538 | 0.407 | | | | 1.25 | 0.315 | 0.323 | 0.319 | 0.188 | | | | 0.625 | 0.226 | 0.241 | 0.234 | 0.103 | | | | 0 | 0.126 | 0.135 | 0.131 | | | |
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货期
3-5 working days