华美生物/CUSABIOPLAU小鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫试剂盒Mouse urokinase plasminogen activator,uPA ELISA kitCSB-E07369m华美-易享

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PLAU小鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫试剂盒Mouse urokinase plasminogen activator,uPA ELISA kit

数万篇文献的选择，荣登各大SCI高分期刊

原料试剂研发实验室

价格

￥2500.00-3800.00

品牌华美生物/CUSABIO

地区中国,湖北省,武汉市

货号 CSB-E07369m

产地国产

选择规格

96T

48T

易享客服

武汉华美生物工程有限公司

湖北省武汉东湖新技术开发区神墩五路269号生之源股份生物科创产业园B栋

营业执照已审核

武汉华美生物工程有限公司成立于2007年12月，是一家以科研试剂生产、诊断试剂原料供应为核心，集自主研发、生产、营销为一体的生物高新技术企业。华美生物拥有多项先进的核心技术，致力于为全球高等院校、政府研究机构、诊断试剂生产厂家以及生物制药公司提供高质量产品与高水平的技术服务。华美生物子品牌CUSABIO主要经营科研试剂产品，现拥有各物种基因产品一万余种；现货重组蛋白16,000多种；数十个种属的多克隆抗体65,000多种和千余种高质量单克隆抗体。ELISA试剂盒现已涵盖20多个种属、3,000个靶标、4,000多种产品，涉及肿瘤、激素、自身免疫、心血管、代谢等众多研究领域。华美CUSABIO产品远销欧美日德等九十多个国家和地区，已被众多国际顶级学术期刊Nature, Cell, Immunity, Nature Medicine, Cell Metabolism, Gastroenterology等引用。华美生物总部位于湖北武汉，在美国建立了子公司，并在全球近百个国家拥有授权经销商。华美生物始终秉承“诚信与品质”的核心理念，借助自身的创新实力，匠心打造精品科研试剂与诊断原料，无畏前行，尽情展现着开创者的梦想与未来，为人类健康事业创造价值！

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产品规格图文详情技术文档

产品规格

品牌名称

华美生物/CUSABIO

货号

CSB-E07369m

国产/进口

国产

规格

96T,48T

图文详情

产品描述

CUSABIO's mouse urokinase plasminogen activator (uPA) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse uPA in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of uPA in samples. The color intensity is measured at 450 nm via a microplate reader.

uPA (PLAU) is a serine protease that cleaves and activates plasminogen, which triggers a proteolytic cascade to regulate extracellular matrix (ECM) proteins. The uPA and uPAR interaction is involved in various cellular activities, including cell proliferation, adhesion, invasion, and survival but is also linked to a broad range of pathological conditions including cancer, atherosclerosis, and kidney disease. In cancer, enhanced levels of the tumor-associated serine protease uPA and its receptor uPAR are linked to tumor progression, metastasis, and shortened survival in patients afflicted with this disease.

别名

PlauUrokinase-type plasminogen activator ELISA kit; U-plasminogen activator ELISA kit; uPA ELISA kit; EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B] ELISA kit

缩写

PLAU

Uniprot No

P06869

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of mouse uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	96-107
1:2	Average %	96
	Range %	90-100
1:4	Average %	95
	Range %	89-100
1:8	Average %	98
	Range %	92-103

回收率

The recovery of mouse uPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	86-97
EDTA plasma (n=4)	101	95-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.535	2.435	2.485	2.322
500	1.918	1.867	1.893	1.730
250	1.170	1.143	1.157	0.994
125	0.641	0.633	0.637	0.474
62.5	0.398	0.385	0.392	0.229
31.2	0.283	0.289	0.286	0.123
15.6	0.209	0.214	0.212	0.049
0	0.166	0.160	0.163

货期

3-5 working days

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