产品描述

This Human SOCS3 ELISA Kit was designed for the quantitative measurement of Human SOCS3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

别名

ATOD4 ELISA Kit; CIS 3 ELISA Kit; CIS-3 ELISA Kit; CIS3 ELISA Kit; Cish3 ELISA Kit; Cytokine induced SH2 protein 3 ELISA Kit; Cytokine-inducible SH2 protein 3 ELISA Kit; E2a Pbx1 target gene in fibroblasts 10 ELISA Kit; EF 10 ELISA Kit; MGC71791 ELISA Kit; SOCS 3 ELISA Kit; SOCS-3 ELISA Kit; Socs3 ELISA Kit; SOCS3_HUMAN ELISA Kit; SSI 3 ELISA Kit; SSI-3 ELISA Kit; SSI3 ELISA Kit; STAT induced STAT inhibitor 3 ELISA Kit; STAT-induced STAT inhibitor 3 ELISA Kit; Suppressor of cytokine signaling 3 ELISA Kit

缩写

SOCS3

Uniprot No

O14543

灵敏度

11.75 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SOCS-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		88-99
1:2		Average %		94
		Range %		82-102
1:4		Average %		91
		Range %		84-105
1:8		Average %		96
		Range %		90-102

回收率

The recovery of human SOCS-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		83-110
EDTA plasma (n=4)		97		86-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   3000 2.069 2.105 2.087 2.002   1500 1.486 1.517 1.502 1.417   750 1.108 1.034 1.071 0.986   375 0.591 0.603 0.597 0.512   187.5 0.396 0.408 0.402 0.317   93.75 0.286 0.274 0.280 0.195   46.88 0.210 0.203 0.207 0.122   0 0.083 0.087 0.085

货期

3-5 working days