产品描述
小鼠TLR4 ELISA试剂盒旨在定量测量血清、血浆和组织匀浆中的小鼠TLR4蛋白。它是一种夹心ELISA试剂盒,检测范围为0.156 ng/mL-10 ng/mL,灵敏度为0.039 ng/mL
别名
Tlr4 ELISA Kit; Lps ELISA Kit; Toll-like receptor 4 ELISA Kit; EC 3.2.2.6 ELISA Kit; CD antigen CD284 ELISA Kit
缩写
TLR4
Uniprot No
Q9QUK6
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse sTLR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
95 |
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| Range % |
88-98 |
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| 1:10 |
Average % |
100 |
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| Range % |
96-103 |
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| 1:20 |
Average % |
94 |
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| Range % |
88-97 |
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| 1:40 |
Average % |
99 |
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| Range % |
95-102 |
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回收率
| The recovery of mouse sTLR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
85-95 |
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| EDTA plasma (n=4) |
102 |
97-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.758 |
2.713 |
2.736 |
2.619 |
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| 5 |
2.012 |
2.087 |
2.050 |
1.933 |
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| 2.5 |
1.204 |
1.178 |
1.191 |
1.074 |
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| 1.25 |
0.663 |
0.652 |
0.658 |
0.541 |
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| 0.625 |
0.378 |
0.366 |
0.372 |
0.255 |
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| 0.312 |
0.275 |
0.281 |
0.278 |
0.161 |
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| 0.156 |
0.194 |
0.187 |
0.191 |
0.074 |
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| 0 |
0.116 |
0.118 |
0.117 |
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货期
3-5 working days