产品描述
This Bovine TGFB1 ELISA Kit was designed for the quantitative measurement of Bovine TGFB1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.
别名
TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写
TGFB1
Uniprot No
P18341
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
99 |
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| Range % |
94-103 |
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| 1:2 |
Average % |
88 |
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| Range % |
83-97 |
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| 1:4 |
Average % |
91 |
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| Range % |
87-95 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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回收率
| The recovery of bovine TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
87-97 |
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| EDTA plasma (n=4) |
103 |
103-109 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.684 |
2.636 |
2.660 |
2.537 |
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| 200 |
2.348 |
2.454 |
2.401 |
2.278 |
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| 100 |
1.786 |
1.884 |
1.835 |
1.712 |
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| 50 |
1.031 |
0.997 |
1.014 |
0.891 |
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| 25 |
0.542 |
0.542 |
0.542 |
0.419 |
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| 12.5 |
0.329 |
0.311 |
0.320 |
0.197 |
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| 6.25 |
0.214 |
0.228 |
0.221 |
0.098 |
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| 0 |
0.119 |
0.126 |
0.123 |
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货期
3-5 working days