产品描述
This Horse TNF ELISA Kit was designed for the quantitative measurement of Horse TNF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
缩写
TNF
Uniprot No
P29553
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of horse TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-97 | |
| 1:2 | Average % | 92 | |
| Range % | 84-99 | |
| 1:4 | Average % | 95 | |
| Range % | 92-103 | |
| 1:8 | Average % | 92 | |
| Range % | 86-98 | |
回收率
| The recovery of horse TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 83-98 | |
| EDTA plasma (n=4) | 94 | 91-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.601 | 2.548 | 2.575 | 2.409 | | | 500 | 2.248 | 2.211 | 2.230 | 2.064 | | | 250 | 1.631 | 1.616 | 1.624 | 1.458 | | | 125 | 0.905 | 0.925 | 0.915 | 0.749 | | | 62.5 | 0.475 | 0.483 | 0.479 | 0.313 | | | 31.2 | 0.312 | 0.321 | 0.317 | 0.151 | | | 15.6 | 0.255 | 0.268 | 0.262 | 0.096 | | | 0 | 0.164 | 0.167 | 0.166 | | |
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货期
3-5 working days