产品描述
The Rat Tumor necrosis factor soluble receptor Ⅰ (TNFsR-Ⅰ) ELISA Kit – a powerful tool for quantitative analysis of the Tumor necrosis factor receptor 1 protein in rat samples.
This ELISA kit is specifically designed for the measurement of TNFsR-Ⅰ protein levels in a variety of sample types, including serum, plasma, and tissue homogenates from Rattus norvegicus (Rat) species. With a detection range of 15.6 pg/mL to 1000 pg/mL and a sensitivity of 3.9 pg/mL, this ELISA kit can accurately detect even low levels of TNFsR-Ⅰ protein.
The assay principle is based on a sandwich immunoassay, which involves the use of two specific antibodies that bind to different epitopes of the target protein. This approach ensures high specificity and sensitivity in the measurement of TNFsR-Ⅰ levels. The assay can be completed in just 1-5 hours, with a sample volume of 50-100ul, making it a fast and convenient option for research. This TNFsR-Ⅰ ELISA kit has been cited in over 2 research articles, demonstrating its reliability and accuracy.
别名
Tnfrsf1a; Tnfr-1; Tnfr1; Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p55; p60; CD antigen CD120a
缩写
TNFsR-
Uniprot No
P22934
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 104 | | |
| Range % | 100-110 | | |
| 1:2 | Average % | 92 | | |
| Range % | 88-96 | | |
| 1:4 | Average % | 98 | | |
| Range % | 94-102 | | |
| 1:8 | Average % | 90 | | |
| Range % | 85-94 | | |
回收率
| The recovery of rat TNFsR-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 87 | 82-93 | | |
| EDTA plasma (n=4) | 95 | 90-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.107 | 2.221 | 2.164 | 1.987 | | | | 500 | 1.562 | 1.499 | 1.531 | 1.354 | | | | 250 | 0.952 | 1.003 | 0.978 | 0.801 | | | | 125 | 0.573 | 0.558 | 0.566 | 0.389 | | | | 62.5 | 0.395 | 0.412 | 0.404 | 0.227 | | | | 31.2 | 0.284 | 0.275 | 0.280 | 0.103 | | | | 15.6 | 0.229 | 0.234 | 0.232 | 0.055 | | | | 0 | 0.181 | 0.173 | 0.177 | | | |
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货期
3-5 working days
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