产品描述
This Human PKM2 ELISA Kit was designed for the quantitative measurement of Human PKM2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 U/mL-60 U/mL and the sensitivity is 0.225 U/mL.
别名
CTHBP ELISA Kit; Cytosolic thyroid hormone-binding protein ELISA Kit; KPYM_HUMAN ELISA Kit; OIP-3 ELISA Kit; Opa-interacting protein 3 ELISA Kit; p58 ELISA Kit; pkm ELISA Kit; PKM1 ELISA Kit; PKM2 ELISA Kit; Pyruvate kinase 2/3 ELISA Kit; Pyruvate kinase muscle isozyme ELISA Kit; Pyruvate kinase PKM ELISA Kit; THBP1 ELISA Kit; Thyroid hormone-binding protein 1 ELISA Kit; Tumor M2-PK ELISA Kit
缩写
PKM2
Uniprot No
P14618
灵敏度
0.225 U/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human M2-PK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 80-95 | |
| 1:2 | Average % | 85 | |
| Range % | 80-93 | |
| 1:4 | Average % | 92 | |
| Range % | 85-98 | |
| 1:8 | Average % | 94 | |
| Range % | 90-100 | |
回收率
| The recovery of human M2-PK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 93-106 | |
| EDTA plasma (n=4) | 90 | 84-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| U/ml | OD1 | OD2 | Average | Corrected | | | 60 | 2.311 | 2.303 | 2.307 | 2.115 | | | 30 | 2.035 | 1.946 | 1.991 | 1.799 | | | 15 | 1.684 | 1.612 | 1.648 | 1.456 | | | 7.5 | 1.323 | 1.297 | 1.310 | 1.118 | | | 3.75 | 0.957 | 0.926 | 0.942 | 0.750 | | | 1.8 | 0.648 | 0.597 | 0.623 | 0.431 | | | 0.9 | 0.312 | 0.300 | 0.306 | 0.114 | | | 0 | 0.196 | 0.188 | 0.192 | | |
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货期
3-5 working days