产品描述
This Human TNFRSF10B ELISA Kit was designed for the quantitative measurement of Human TNFRSF10B protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
TNFRSF10B; DR5; KILLER; TRAILR2; TRICK2; ZTNFR9; UNQ160/PRO186; Tumor necrosis factor receptor superfamily member 10B; Death receptor 5; TNF-related apoptosis-inducing ligand receptor 2; TRAIL receptor 2; TRAIL-R2; CD antigen CD262
缩写
TNFRSF10B
Uniprot No
O14763
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRAIL-R2/DR5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
81-89 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-94 |
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| 1:4 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:8 |
Average % |
105 |
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| Range % |
101-109 |
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回收率
| The recovery of human TRAIL-R2/DR5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
95-103 |
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| EDTA plasma (n=4) |
86 |
83-89 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.604 |
2.598 |
2.601 |
2.514 |
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| 1000 |
2.293 |
2.204 |
2.249 |
2.162 |
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| 500 |
1.687 |
1.658 |
1.673 |
1.586 |
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| 250 |
1.087 |
1.002 |
1.045 |
0.958 |
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| 125 |
0.629 |
0.638 |
0.634 |
0.547 |
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| 62.5 |
0.454 |
0.468 |
0.461 |
0.374 |
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| 31.25 |
0.247 |
0.231 |
0.239 |
0.152 |
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| 0 |
0.085 |
0.089 |
0.087 |
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货期
3-5 working days