产品描述
CUSABIO's human syndecan-1 (SDC1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for quantitatively measuring human SDC1 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of SDC1 in samples. The color intensity is measured at 450 nm via a microplate reader.
SDC1, alternatively referred to as CD138, is a heparan sulfate proteoglycan that is expressed predominantly on epithelia and leukocytes and is involved in cell proliferation, cell growth, metastasis, angiogenesis, cell-extracellular matrix interactions, inflammatory response, and wound healing. It can bind to integrins, growth factors, cytokines as well as chemokines. SDC1 plays a major role in the clearance of triglycerides. It is implicated in the differentiation and prognosis of various tumors. Dysregulated expression of SDC1 has been found in different cancer types and contributes to cancer progression by promoting cell proliferation, metastasis, invasion, and angiogenesis, and is associated with relapse through chemoresistance.
别名
CD_antigen ELISA Kit; CD138 ELISA Kit; CD138 antigen ELISA Kit; heparan sulfate proteoglycan fibroblast growth factor receptor ELISA Kit; SDC ELISA Kit; Sdc1 ELISA Kit; SDC1_HUMAN ELISA Kit; SYND1 ELISA Kit; Syndecan 1 ELISA Kit; Syndecan ELISA Kit; syndecan proteoglycan 1 ELISA Kit; Syndecan-1 ELISA Kit
缩写
SDC1
Uniprot No
P18827
灵敏度
1.17 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 82-91 | |
| 1:2 | Average % | 96 | |
| Range % | 91-101 | |
| 1:4 | Average % | 94 | |
| Range % | 88-99 | |
| 1:8 | Average % | 95 | |
| Range % | 90-100 | |
回收率
| The recovery of human SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 93-99 | |
| EDTA plasma (n=4) | 90 | 84-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 300 | 2.439 | 2.411 | 2.425 | 2.272 | | | 150 | 2.048 | 2.001 | 2.025 | 1.872 | | | 75 | 1.635 | 1.547 | 1.591 | 1.438 | | | 37.5 | 1.182 | 1.106 | 1.144 | 0.991 | | | 18.75 | 0.843 | 0.781 | 0.812 | 0.659 | | | 9.4 | 0.467 | 0.438 | 0.453 | 0.300 | | | 4.7 | 0.282 | 0.274 | 0.278 | 0.125 | | | 0 | 0.159 | 0.147 | 0.153 | | |
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货期
3-5 working days
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