产品描述
This Mouse SFTPB ELISA Kit was designed for the quantitative measurement of Mouse SFTPB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.
别名
Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
缩写
SFTPB
Uniprot No
P50405
灵敏度
0.11 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 86-97 | |
| 1:2 | Average % | 97 | |
| Range % | 92-102 | |
| 1:4 | Average % | 88 | |
| Range % | 85-93 | |
| 1:8 | Average % | 89 | |
| Range % | 83-94 | |
回收率
| The recovery of mouse SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 87-96 | |
| EDTA plasma (n=4) | 102 | 96-109 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 30 | 2.176 | 2.206 | 2.191 | 2.031 | | | 15 | 1.511 | 1.544 | 1.528 | 1.368 | | | 7.5 | 0.897 | 0.970 | 0.934 | 0.774 | | | 3.75 | 0.612 | 0.618 | 0.615 | 0.455 | | | 1.8 | 0.362 | 0.392 | 0.377 | 0.217 | | | 0.9 | 0.279 | 0.267 | 0.273 | 0.113 | | | 0.45 | 0.225 | 0.220 | 0.223 | 0.063 | | | 0 | 0.156 | 0.163 | 0.160 | | |
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货期
3-5 working days