产品描述

This Human SERPINB2 ELISA Kit was designed for the quantitative measurement of Human SERPINB2 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

HsT1201 ELISA Kit; Monocyte Arg serpin ELISA Kit; Monocyte Arg-serpin ELISA Kit; Monocyte Arginine-serpin ELISA Kit; Monocyte-derived plasminogen activator inhibitor ELISA Kit; PAI ELISA Kit; PAI-2 ELISA Kit; PAI2 ELISA Kit; PAI2_HUMAN ELISA Kit; Placental plasminogen activator inhibitor ELISA Kit; PLANH2 ELISA Kit; Plasminogen activator inhibitor 2 ELISA Kit; plasminogen activator inhibitor; type 2 ELISA Kit; plasminogen activator inhibitor; type II (arginine-serpin) ELISA Kit; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 2 ELISA Kit; Serpin B2 ELISA Kit; serpin peptidase inhibitor; clade B (ovalbumin); member 2 ELISA Kit; Serpinb2 ELISA Kit; Urokinase inhibitor ELISA Kit

缩写

SERPINB2

Uniprot No

P05120

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:200		Average %		96
		Range %		90-102
1:400		Average %		98
		Range %		93-104
1:800		Average %		95
		Range %		89-99
1:1600		Average %		88
		Range %		84-92

回收率

The recovery of human SERPINB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		99		95-103
EDTA plasma (n=4)		86		82-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.687 2.521 2.604 2.455 500 2.126 2.235 2.181 2.032 250 1.586 1.573 1.580 1.431 125 0.972 0.995 0.984 0.835 62.5 0.617 0.606 0.612 0.463 31.25 0.378 0.365 0.372 0.223 15.6 0.232 0.237 0.235 0.086 0 0.149 0.148 0.149

货期

3-5 working days