产品描述
This human SFRP4 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SFRP4 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the SFRP4 levels in the sample and is measured at 450 nm through a microplate reader.
SFRP4 is expressed in the intestinal tract, endometrium, pancreas, stomach, liver, heart, and mammary gland. Ample studies have shown that SFRP4 regulates apoptosis and differentiation of tumor cells and is an important antagonist of the Wnt pathway. Loss of SFRP4 expression has been observed in most tumors, including ovarian cancer, malignant pituitary tumors, endometrial cancer, and acute myeloid leukemia. However, upregulation of SFRP4 is found in chemotherapy-sensitive ovarian cancer and breast cancer. Additionally, SFRP4 is implicated in many diseases including obesity, type 2 diabetes (T2D), and cancer.
别名
Frizzled protein ELISA Kit; Frizzled protein human endometrium ELISA Kit; FRP 4 ELISA Kit; FRP4 ELISA Kit; FrpHE ELISA Kit; human endometrium ELISA Kit; PYL ELISA Kit; Secreted frizzled-related protein 4 ELISA Kit; sFRP-4 ELISA Kit; Sfrp4 ELISA Kit; SFRP4_HUMAN ELISA Kit
缩写
SFRP4
Uniprot No
Q6FHJ7
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Stem Cells
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SFRP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 95 | |
| Range % | 91-99 | |
| 1:10 | Average % | 100 | |
| Range % | 95-105 | |
| 1:20 | Average % | 92 | |
| Range % | 88-97 | |
| 1:40 | Average % | 88 | |
| Range % | 82-92 | |
回收率
| The recovery of human SFRP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 83-95 | |
| EDTA plasma (n=4) | 99 | 94-103 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.712 | 2.652 | 2.682 | 2.570 | | | 50 | 1.885 | 1.776 | 1.831 | 1.719 | | | 25 | 1.212 | 1.204 | 1.208 | 1.096 | | | 12.5 | 0.621 | 0.664 | 0.643 | 0.531 | | | 6.25 | 0.383 | 0.357 | 0.370 | 0.258 | | | 3.12 | 0.297 | 0.278 | 0.288 | 0.176 | | | 1.56 | 0.209 | 0.212 | 0.211 | 0.099 | | | 0 | 0.116 | 0.107 | 0.112 | | |
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
