产品描述

This Sheep TNF ELISA Kit was designed for the quantitative measurement of Sheep TNF protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.125 pg/mL-50 pg/mL and the sensitivity is 3.125 pg/mL.

别名

TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit

缩写

TNF

Uniprot No

P23383

灵敏度

3.125 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of sheep TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-88
1:2		Average %		95
		Range %		90-99
1:4		Average %		90
		Range %		85-94
1:8		Average %		102
		Range %		98-109

回收率

The recovery of sheep TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		84-95
EDTA plasma (n=4)		96		90-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average     50 0.161 0.165 0.163     25 0.308 0.324 0.316     12.5 0.551 0.512 0.532     6.25 1.012 0.982 0.997     3.125 2.054 2.011 2.033

货期

3-5 working days