产品描述

This Human SULT1A1 ELISA Kit was designed for the quantitative measurement of Human SULT1A1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

Aryl sulfotransferase 1 ELISA Kit; HAST1/HAST2 ELISA Kit; Human aryl sulfotransferase mRNA complete cds ELISA Kit; MGC131921 ELISA Kit; MGC5163 ELISA Kit; OTTHUMP00000162568 ELISA Kit; OTTHUMP00000162569 ELISA Kit; P PST 1 ELISA Kit; P PST ELISA Kit; P-PST 1 ELISA Kit; Phenol sulfating phenol sulfotransferase 1 ELISA Kit; Phenol sulfotransferase 1 ELISA Kit; Phenol-sulfating phenol sulfotransferase 1 ELISA Kit; PST ELISA Kit; ST1A1 ELISA Kit; ST1A1_HUMAN ELISA Kit; ST1A3 ELISA Kit; STP ELISA Kit; STP1 ELISA Kit; Sulfotransferase 1A1 ELISA Kit; Sulfotransferase family 1A phenol preferring member 1 ELISA Kit; Sulfotransferase family cytosolic 1A phenol preferring member 1 ELISA Kit; Sulfotransferase phenol preferring 1 ELISA Kit; SULT1A1 ELISA Kit; Thermostable phenol sulfotransferase ELISA Kit; Thermostable phenol sulfotransferase1 ELISA Kit; Ts-PST ELISA Kit; TSPST1 ELISA Kit

缩写

SULT1A1

Uniprot No

P50225

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SULT1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-89
1:2		Average %		103
		Range %		98-107
1:4		Average %		88
		Range %		84-92
1:8		Average %		98
		Range %		94-102

回收率

The recovery of human SULT1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		85-95
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1600 2.078 2.148 2.113 1.918 800 1.512 1.468 1.490 1.295 400 1.004 1.020 1.012 0.817 200 0.733 0.689 0.711 0.516 100 0.496 0.511 0.504 0.309 50 0.381 0.374 0.378 0.183 25 0.275 0.286 0.281 0.086 0 0.197 0.192 0.195

货期

3-5 working days