产品描述
The Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA kit is the ultimate tool for researchers seeking to quantify SRD5A2 levels in samples of rat serum, plasma, tissue homogenates, and cell lysates.
The SRD5A2 protein, also known as 3-oxo-5-alpha-steroid 4-dehydrogenase 2, is involved in processing androgens, which are hormones that direct male sexual development,making it an essential protein for researchers in this field. Our ELISA kit is designed to provide fast, accurate, and reliable results, allowing researchers to measure SRD5A2 levels with high sensitivity and precision.
With a detection range of 6 pg/mL to 1200 pg/mL and a sensitivity of 3 pg/mL, this ELISA kit delivers precise and accurate results. The assay time is just 1-5 hours, and the sample volume required is only 50-100ul, making it a highly efficient tool for researchers.
The kit uses a competitive assay principle, enabling quantitative measurement of SRD5A2 levels in samples. The detection wavelength is 450 nm, making it easy to read and interpret results.
别名
Srd5a2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2
缩写
SRD5A2
Uniprot No
P31214
灵敏度
3 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-99 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-103 |
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| 1:8 |
Average % |
90 |
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| Range % |
87-94 |
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回收率
| The recovery of rat SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
95-101 |
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| EDTA plasma (n=4) |
93 |
87-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 1200 |
0.225 |
0.217 |
0.221 |
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| 300 |
0.411 |
0.434 |
0.423 |
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| 96 |
0.651 |
0.632 |
0.642 |
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| 24 |
1.055 |
1.017 |
1.036 |
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| 6 |
1.497 |
1.458 |
1.478 |
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| 0 |
2.359 |
2.450 |
2.405 |
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货期
3-5 working days
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