产品描述
This Rat PF4 ELISA Kit was designed for the quantitative measurement of Rat PF4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.
别名
Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit
缩写
PF4
Uniprot No
P06765
灵敏度
0.039 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 86-97 | |
| 1:2 | Average % | 95 | |
| Range % | 88-99 | |
| 1:4 | Average % | 95 | |
| Range % | 91-102 | |
| 1:8 | Average % | 95 | |
| Range % | 87-104 | |
回收率
| The recovery of rat PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 83-99 | |
| EDTA plasma (n=4) | 97 | 89-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| μg/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.684 | 2.617 | 2.651 | 2.463 | | | 5 | 2.204 | 2.186 | 2.195 | 2.007 | | | 2.5 | 1.563 | 1.608 | 1.586 | 1.398 | | | 1.25 | 0.881 | 0.899 | 0.890 | 0.702 | | | 0.625 | 0.480 | 0.491 | 0.486 | 0.298 | | | 0.312 | 0.345 | 0.337 | 0.341 | 0.153 | | | 0.156 | 0.247 | 0.250 | 0.249 | 0.061 | | | 0 | 0.185 | 0.190 | 0.188 | | |
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货期
3-5 working days