产品描述
RuBisCO(Ribulose-1,5-bisphosphate carboxylase/oxygenase)中文名为核糖骨架1,5-双磷酸羧化酶/氧化酶,是光合作用的关键酶之一,催化二氧化碳和核糖骨架1,5-双磷酸的反应,进而固定CO2为有机物。RuBisCO含量是衡量植物光合产物的重要指标,也是地球上最丰富的酶之一。
华美生物所提供的Plant Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测植物 (鼠-水芹)植物组织样本中的RuBisCO,其灵敏度为3.12 μg/mL,检测范围为3.12 μg/mL-800 μg/mL。
缩写
RuBisCO
灵敏度
3.12 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<10% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<20% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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回收率
| The recovery of plant RuBisCO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| leaf tissue (n=5) |
95 |
89-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 800 |
0.047 |
0.050 |
0.049 |
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| 200 |
0.261 |
0.268 |
0.265 |
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| 50 |
0.467 |
0.473 |
0.470 |
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| 12.5 |
0.670 |
0.682 |
0.676 |
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| 3.12 |
1.139 |
1.143 |
1.141 |
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| 0 |
1.861 |
1.916 |
1.889 |
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货期
3-5 working days