产品描述

This Human PROS1 ELISA Kit was designed for the quantitative measurement of Human PROS1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

别名

Preproprotein S ELISA Kit; Propiece of latent protein S ELISA Kit; PROS 1 ELISA Kit; PROS ELISA Kit; PROS_HUMAN ELISA Kit; proS1 ELISA Kit; Protein S alpha ELISA Kit; Protein Sa ELISA Kit; PS 21 ELISA Kit; PS 22 ELISA Kit; PS 23 ELISA Kit; PS 24 ELISA Kit; PS 25 ELISA Kit; PS 26 ELISA Kit; PS21 ELISA Kit; PS22 ELISA Kit; PS23 ELISA Kit; PS24 ELISA Kit; PS25 ELISA Kit; PS26 ELISA Kit; PSA ELISA Kit; THPH5 ELISA Kit; THPH6 ELISA Kit; Vitamin K dependent protein S ELISA Kit; Vitamin K-dependent plasma protein S ELISA Kit; Vitamin K-dependent protein S ELISA Kit

缩写

Protein S

Uniprot No

P07225

灵敏度

0.78 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Protein S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		91
		Range %		87-95
1:200		Average %		95
		Range %		90-100
1:400		Average %		97
		Range %		94-101
1:800		Average %		94
		Range %		91-98

回收率

The recovery of human Protein S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-94
EDTA plasma (n=4)		99		93-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 200 2.955 3.003 2.979 2.874 100 2.134 2.254 2.194 2.089 50 1.460 1.568 1.514 1.409 25 0.788 0.792 0.790 0.685 12.5 0.436 0.469 0.453 0.348 6.25 0.265 0.254 0.260 0.155 3.12 0.177 0.166 0.172 0.067 0 0.101 0.109 0.105

货期

3-5 working days