产品描述

This Human PTK7 ELISA Kit was designed for the quantitative measurement of Human PTK7 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.125 pg/mL.

别名

8430404F20Rik ELISA Kit; CCK 4 ELISA Kit; CCK-4 ELISA Kit; Colon carcinoma kinase 4 ELISA Kit; Human colon carcinoma kinase 4 (CCK4) mRNA complete cds ELISA Kit; Inactive tyrosine-protein kinase 7 ELISA Kit; mPTK7/CCK4 ELISA Kit; OTTHUMP00000217016 ELISA Kit; Protein tyrosine kinase PTK 7 ELISA Kit; Protein-tyrosine kinase 7 ELISA Kit; Pseudo tyrosine kinase receptor 7 ELISA Kit; PTK 7 ELISA Kit; PTK7 ELISA Kit; PTK7 protein tyrosine kinase 7 ELISA Kit; PTK7 protein tyrosine kinase ELISA Kit; PTK7_HUMAN ELISA Kit; Serum response factor ELISA Kit; Srf ELISA Kit; Tyrosine-protein kinase-like 7 ELISA Kit

缩写

PTK7

Uniprot No

Q13308

灵敏度

3.125 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PTK7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		84-95
1:2		Average %		92
		Range %		87-101
1:4		Average %		98
		Range %		94-104
1:8		Average %		98
		Range %		93-105

回收率

The recovery of human PTK7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		93-102
EDTA plasma (n=4)		91		84-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     800 2.274 2.265 2.270 2.161     400 1.779 1.812 1.796 1.687     200 1.134 1.158 1.146 1.037     100 0.660 0.712 0.686 0.577     50 0.385 0.410 0.398 0.289     25 0.233 0.235 0.234 0.125     12.5 0.174 0.187 0.181 0.072     0 0.107 0.110 0.109

货期

3-5 working days