产品描述
S100B,全称S100 calcium-binding protein B,是一种钙结合蛋白,主要在神经系统中表达。S100B可以调节神经元的增殖和存活,对神经发育、神经保护和神经炎症等生理和病理过程具有重要作用。此外,S100B还参与了肿瘤、心血管和炎症等多种疾病的发生和发展。在生理状态下,S100B的含量非常低,而在某些病理情况下,如脑损伤、炎症和肿瘤,其含量会显著升高,因此S100B被广泛应用于相关疾病的诊断和治疗。
华美生物所提供的Rat Soluble protein-100B,S-100B ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的S100B,其灵敏度为0.78 pg/mL,检测范围为3.12 pg/mL-200 pg/mL。
别名
S100b ELISA Kit; Protein S100-B ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 calcium-binding protein B ELISA Kit
缩写
S100B
Uniprot No
P04631
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-93 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-104 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-101 |
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回收率
| The recovery of rat S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-96 |
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| EDTA plasma (n=4) |
89 |
84-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
1.954 |
1.874 |
1.914 |
1.751 |
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| 100 |
1.562 |
1.531 |
1.547 |
1.384 |
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| 50 |
1.174 |
1.168 |
1.171 |
1.008 |
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| 25 |
0.802 |
0.816 |
0.809 |
0.646 |
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| 12.5 |
0.567 |
0.552 |
0.560 |
0.397 |
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| 6.25 |
0.347 |
0.355 |
0.351 |
0.188 |
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| 3.12 |
0.264 |
0.261 |
0.263 |
0.100 |
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| 0 |
0.158 |
0.167 |
0.163 |
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货期
3-5 working days