产品描述
This human spondin-1 (SPON1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SPON1 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the SPON1 levels in the sample and is measured at 450 nm through a microplate reader.
SPON1 is an extracellular matrix protein that is derived from the floor plate of vertebrate embryos and is involved in the growth of axons during embryonic development, differentiation of neural cells, and the promotion of axonal regeneration post-injury. SPON1 could bind the amyloid precursor protein and inhibit its cleavage by β-secretase, leading to cognitive decline in Alzheimer's disease. Aberrant expression of SPON1 has been found in solid tumors. SPON1 has been reported to promote tumor invasion and metastasis in liver cancer.
别名
SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit
缩写
SPON1
Uniprot No
Q9HCB6
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 84 | |
| Range % | 80-91 | |
| 1:2 | Average % | 86 | |
| Range % | 81-92 | |
| 1:4 | Average % | 92 | |
| Range % | 87-100 | |
| 1:8 | Average % | 95 | |
| Range % | 88-101 | |
回收率
| The recovery of human spondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 90-104 | |
| EDTA plasma (n=4) | 93 | 86-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.504 | 2.463 | 2.484 | 2.365 | | | 5 | 2.006 | 2.042 | 2.024 | 1.905 | | | 2.5 | 1.506 | 1.479 | 1.493 | 1.374 | | | 1.25 | 0.973 | 0.897 | 0.935 | 0.816 | | | 0.625 | 0.511 | 0.518 | 0.515 | 0.396 | | | 0.312 | 0.285 | 0.268 | 0.276 | 0.157 | | | 0.156 | 0.176 | 0.187 | 0.182 | 0.063 | | | 0 | 0.121 | 0.117 | 0.119 | | |
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货期
3-5 working days
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