产品描述

This Human PEBP1 ELISA Kit was designed for the quantitative measurement of Human PEBP1 protein in serum, plasma, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

Epididymis luminal protein 210 ELISA Kit; Epididymis secretory protein Li 34 ELISA Kit; Epididymis secretory protein Li 96 ELISA Kit; HCNP ELISA Kit; HCNPpp ELISA Kit; HEL 210 ELISA Kit; HEL S 34 ELISA Kit; HEL S 96 ELISA Kit; Hippocampal cholinergic neurostimulating peptide ELISA Kit; Neuropolypeptide h3 ELISA Kit; PBP ELISA Kit; PEBP ELISA Kit; PEBP 1 ELISA Kit; PEBP-1 ELISA Kit; Pebp1 ELISA Kit; PEBP1_HUMAN ELISA Kit; Phosphatidylethanolamine binding protein ELISA Kit; Phosphatidylethanolamine binding protein 1 ELISA Kit; Prostatic binding protein ELISA Kit; Prostatic-binding protein ELISA Kit; R kip ELISA Kit; Raf kinase inhibitor protein ELISA Kit; Raf kinase inhibitory protein ELISA Kit; RKIP ELISA Kit

缩写

PEBP1

Uniprot No

P30086

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PEBP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		102
		Range %		89-107
1:2		Average %		101
		Range %		96-105
1:4		Average %		94
		Range %		87-98
1:8		Average %		94
		Range %		87-99

回收率

The recovery of human PEBP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		102		93-106
EDTA plasma (n=4)		101		95-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 10 2.483 2.423 2.453 2.308 5 1.902 1.876 1.889 1.744 2.5 1.271 1.245 1.258 1.113 1.25 0.853 0.802 0.828 0.683 0.625 0.481 0.471 0.476 0.331 0.312 0.336 0.328 0.332 0.187 0.156 0.241 0.234 0.238 0.093 0 0.148 0.142 0.145

货期

3-5 working days