产品描述
The Rat Runt-related transcription factor 2 (RUNX2) ELISA kit is a powerful tool for the quantitative measurement of RUNX2 protein levels in serum, plasma, tissue homogenates, and cell lysates of Rattus norvegicus (Rat) samples.
RUNX2, also known as CBFA1, is a transcription factor that plays a critical role in bone development and remodeling，signal pathway. Our ELISA kit is specifically designed for the measurement of RUNX2 protein levels, making it an ideal tool for studying the molecular mechanisms underlying bone formation and skeletal disorders in rats.
With a detection range of 3.12 ng/mL to 200 ng/mL and a sensitivity of 0.78 ng/mL, our RUNX2 ELISA kit offers exceptional accuracy and precision in measuring protein levels. The assay time is only 1-5 hours, and the sample volume required is just 50-100ul. The detection wavelength is 450 nm, and the assay principle is quantitative, utilizing a sandwich method for precise measurements.
Our RUNX2 ELISA kit is suitable for researchers studying epigenetics and nuclear signaling in the context of bone development and disease. It is user-friendly and easy to use, providing reliable results for the research applications.
别名
Runx2 ELISA Kit; Cbfa1 ELISA Kit; Osf2 ELISA Kit; Runt-related transcription factor 2 ELISA Kit; Core-binding factor subunit alpha-1 ELISA Kit; CBF-alpha-1 ELISA Kit; Osteoblast-specific transcription factor 2 ELISA Kit; OSF-2 ELISA Kit; Fragments ELISA Kit
缩写
RUNX2
Uniprot No
Q9Z2J9
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat RUNX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
98 |
|
| Range % |
91-103 |
|
| 1:2 |
Average % |
102 |
|
| Range % |
94-107 |
|
| 1:4 |
Average % |
88 |
|
| Range % |
83-91 |
|
| 1:8 |
Average % |
94 |
|
| Range % |
84-98 |
|
回收率
| The recovery of rat RUNX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
103 |
93-107 |
|
| EDTA plasma (n=4) |
98 |
90-102 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 200 |
2.551 |
2.452 |
2.502 |
2.322 |
|
| 100 |
2.126 |
1.996 |
2.061 |
1.881 |
|
| 50 |
1.359 |
1.341 |
1.350 |
1.170 |
|
| 25 |
0.793 |
0.818 |
0.806 |
0.626 |
|
| 12.5 |
0.474 |
0.481 |
0.478 |
0.298 |
|
| 6.25 |
0.395 |
0.403 |
0.399 |
0.219 |
|
| 3.12 |
0.266 |
0.257 |
0.262 |
0.082 |
|
| 0 |
0.182 |
0.178 |
0.180 |
|
|
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
