产品描述
This Bovine TAT-III ELISA Kit was designed for the quantitative measurement of Bovine TAT-III protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.78 pg/mL.
缩写
TAT-III
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine TAT-III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:20 |
Average % |
92 |
|
|
| Range % |
88-96 |
|
|
| 1:40 |
Average % |
85 |
|
|
| Range % |
80-90 |
|
|
| 1:80 |
Average % |
104 |
|
|
| Range % |
100-110 |
|
|
| 1:160 |
Average % |
97 |
|
|
| Range % |
92-101 |
|
|
回收率
| The recovery of bovine TAT-III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
95 |
90-100 |
|
|
| EDTA plasma (n=4) |
89 |
85-93 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| pg/ml |
OD1 |
OD2 |
Average |
|
|
|
| 100 |
0.159 |
0.163 |
0.161 |
|
|
|
| 50 |
0.279 |
0.267 |
0.273 |
|
|
|
| 25 |
0.455 |
0.471 |
0.463 |
|
|
|
| 12.5 |
0.724 |
0.704 |
0.714 |
|
|
|
| 6.25 |
1.071 |
1.006 |
1.039 |
|
|
|
| 3.12 |
1.279 |
1.322 |
1.301 |
|
|
|
| 1.56 |
1.683 |
1.718 |
1.701 |
|
|
|
| 0 |
2.106 |
2.235 |
2.171 |
|
|
|
|
货期
3-5 working days